Nutraculture™ Phycocyanin™ is a natural dietary supplement dedicated to assisting individuals who are undergoing cancer therapies including chemotherapy and radiation. Besides, it also has cancer fighting and cancer preventive attributes of its own. Nutraculture™ Phycocyanin™ is a research innovation introduced by our team of world class researchers who harnessed the power of this wonder molecule from Spirulina platensis, one of the oldest species on this planet. Our advanced high through put technology evolving from the combination of the latest medical research and the hidden powers of the natural microalgae result in this specialized anticancer dietary supplement that is a promising support in cancer fight. Nutraculture™ Phycocyanin™ may be used to prepare for and ease negative symptoms during treatment as well as help the body revive and rejuvenate post treatment. Nutraculture™ Phycocyanin™ is being presented with the essential levels of purity and consistency to combat cancer as well as other related medical complications arising due to cancer onset and to meet the unique needs of cancer prevention attributes.
Nutraculture™ Phycocyanin™ is an important molecule extracted from Spirulina platensis, a 3.6 billion years old known microalgae. It is a natural, water soluble and non-toxic molecule with potent anti-cancer, anti oxidant and anti-inflammatory properties. Various research studies also support strong cytoprotective, hepatoprotective and neuroprotective profile of phycocyanin. (Chen and Wong 2008; Romay et. al., 2003; Vadiraja et. al., 1998). This natural molecule is an innovative revolution not only to address the unique health care needs of newly diagnosed cancer patients and patients already undergoing cancer treatment (including chemotherapies , radiotherapies etc) but is also a cancer preventive measure. For many cancer patients the side effects of treatments have an adverse impact on their health and immunity, often making it difficult for them to continue with strong medical therapies. Its unique anti-cancer and cancer preventive profile is based on research proven scientific accreditations.
WHAT DOES NUTRACULTURE™ PHYCOCYANIN™ CONTAIN? Nutraculture™ Phycocyanin™ has 100% natural C-Phycocyanin.
In the current times where dissipated lifestyle and other counting factors such as dietary, physical, mental mismanagements, drug administration intoxicifications in the form of chemotherapies and chemical toxicities are adding up to the cumulative risk of cancer occurrence, consumption of Nutraculture™ Phycocyanin™ is essentially required. Nutraculture™ Phycocyanin™ helps fight and revive against cancer and address other similar conditions which may deteriorate the quality of health. It provides protection and vitality to the body by boosting the immune system. Due to its strong anti-oxidant properties it detoxifies and rejuvenates the overall health profile.
Phycocyanin is naturally designed to meet the unique needs of cancer prevention attributes and is being presented with the essential levels of purity and consistency. Zero toxicity and accomplish safety profiles of this wonder molecule, make it a choice of health conscious individuals as well as cancer fighting subjects without any hesitation.
Cancer is the uncontrolled growth of cells in the body, which is caused by the stepwise accumulation of mutations that affect cell growth control, differentiation and survival. Growth of cancer cells is regulated by the balance between cell proliferation and apoptosis, which is a normal physiological process serving to eliminate unwanted cells and maintain homeostasis in healthy tissue. Hence inducing apoptosis is a desired mode of treating cancer (Tansuwanwong et. al., 2006).
Nutraculture™ Phycocyanin™ induces apoptosis in the existing and proliferating cancer cells and being a natural antioxidant, it also helps getting rid of any cancer promoting oxidative stress. Phycocyanin lowers the amount of cyclooxygenase-2 which is up regulated in cancer cells. It is established that Phycocyanin induces apoptosis in cancer cells by changing the Bcl-2/Bax ratio (Bcl-2 is an anti apoptotic protein, Bax is a pro-apoptotic protein, the ratio of Bcl-2/Bax represents the degree of apoptosis) and the release of Cyt-c in the cytosol (Lu et. al., 2011).
Cancer Therapy by inducing Apoptosis:
Nutraculture™ Phycocyanin™ is one of its kind natural molecule, which has an established promising value as a cancer preventive moiety. In addition to its anti cancerous & apoptotic effects, its free radical scavenging ability or Cancer preventive antioxidant capacity is also one important asset (Romay et. al., 1998). Many problems that affect health and well being are caused by oxidative stress, which is characterized by excessive formation of ROS (Reactive Oxygen Species) that cannot be counteracted by one’s normal antioxidant defense system. In order to protect the body against the consequences of oxidative stress, a successful approach consists of improving one’s antioxidant profile is needed. Therefore adding additional natural antioxidants is helpful to prevent free radical-induced cell damage.Nutraculture™ Phycocyanin™ is able to scavenge free radicals in the cells, protecting cells from damage which prevents or lessens the severity of a disease (Wang et. al., 2007). Studies have shown that phycocyanin is an efficient scavenger of oxygen free radicals (Bhat et. al., 2001) and also reacts with other oxidants of pathological relevance such as HOCl and ONOO- thus proving that the therapeutic use of phycocyanin appears to be promising.
It’s easy digestibility and water solubility, make it a possible natural agent for reducing the harmful effects of oxidative stress without any risk or toxicity.
A further reason for using Phycocyanin in cancer therapy is that the regular intakes of this molecule have been shown to boost immune responses. The particular types of immune cells involved in cancer control, cytotoxic T lymphocytes and monocytes, function more effectively with increased intakes of phycocyanin (Abd-EI BHH and EI-Baroty GS, 2013). Therefore, the likely benefits of Phycocyanin™ in clinical cancer therapy especially when used as an adjuvant to chemotherapy are:
One of the most widely studied effects of Phycocyanin is its antioxidant capacity and its free radical scavenging ability, both in-vivo & in-vitro (Romay et. al., 1998). Anti-oxidant potential of phycocyanin is mainly attributed to its phycobilisome (chromophore) moiety (Patel et. al., 2006) and partially to its apoprotein counterpart (Apt et. al., 1995) as the former shows high degree of conjugation of double bonds which stabilizes free radicals. It is well known that reactive oxygen species (ROS) are involved in a diversity of important processes in medicine including inflammation, atherosclerosis, cancer, reperfusion injury etc. One way by which a substance can interfere with these processes, is by acting as an antioxidant. Phycocyanin is able to scavenge free radicals in the cells, protecting cells from damage which prevents or lessens the severity of the diseases (Wang et. al., 2007).
Inflammation is the body's attempt at self-protection; the aim being to remove harmful stimuli, including damaged cells, irritants, or pathogens - and begin the healing process. characterized by heat, redness, swelling, and pain.
Phycocyanin has a preventive effect against inflammation by following mechanisms (Pardhasaradhi et al., 2000; (Romay et al., 2003; Deng and Chow, 2011; Joventino et al., 2012).
A further reason for using phycocyanin in cancer therapy is that the regular intakes of this molecule have been shown to boost immune responses. Therefore, the likely benefits of PHYCOCYANIN™ in clinical cancer therapy especially when used as an adjuvant to chemotherapy are: improved response of cancers to chemotherapy, boost immune system to fight cancer spread; and reduced risk that chemotherapy may eventually give rise to a new cancer.
Lipid peroxidation mediated by ROS is believed to be an important cause of destruction and damage to cell membranes. Research suggests that Phycocyanin is selective COX-2 inhibitor (Reddy et al., 2000), which significantly inhibits liver microsomal lipid peroxidation thus protecting the liver by preventing oxidative stress in hepatocytes acting as hepatoprotective molecule (Sathyasaikumar et al., 2007). Phycocyanin also inhibits microsomal lipid peroxidation induced by Fe+2 – ascorbic acid or the free radical initiator 2, 2’ azobis (2-amidinopropane) hydrochloride (AAPH) (Bermejo-Bescos et al 2008). Furthermore, it reduces carbon tetrachloride (CCl4)- induced lipid peroxidation. The inhibition of COX-2 by phycocyanin is also known to be involved in its hepatoprotective effect on CCl4-induced liver damage.
Recent studies show that immune-modulatory properties, anti-inflammatory and antioxidant activities contribute to the neuroprotective effects of Phycocyanin. A scientific study demonstrates that either the prophylactic or the therapeutic application of Phycocyanin was able to significantly reduce the infarct volume, and also protect hippocampal neurons from death, induced by global cerebral ischemia/ reperfusion injury in gerbils (Penton-Rol et al, 2011). Studies reveal that Phyocyanin is a potent platelet aggregation inhibitor with a potential to hamper arterial thromboembolism in conjunction with its neuroprotective ability.
Nutraculture™ Phycocyanin™ protects the integrity of the renal cell by stabilizing the free radical mediated LPO and protect against oxalate induced nephro injury (Farooq et al., 2004). Oxalate causes its deleterious effects to kidneys, liver and the hematological system by inducing oxidative stress, which further leads to membrane integrity loss, renal cell damage and, finally, calcium oxalate crystal deposition. Lipid peroxidation (LPO) produce a great variety of stable, diffusible saturated and unsaturated aldehydes like malondialdehyde (MDA) that are extremely active and can diffuse within or even escape from the cell and attack targets far from the site of the original free radical initiated event, resulting in cell damage and therefore act as ‘cytotoxic second messengers'. Phycocyanin pre-treatment decreased the LPO and reversed the effects of oxalate on oxidative stress parameters by interacting with hydroxyl radical and by rebalancing the GSH content, catalase and G6PD activity in oxalate treated animals (Farooq et al., 2006).
Cardioprotective effect of Phycocyanin against ischemia-reperfusion (I/R)-induced myocardial injury in an isolated perfused Langendorff heart model was observed via modulation of p38 MAPK and ERK1/2 pathways (Khan et al., 2006). Another studies reports the possible role of the antioxidant nature of C-phycocyanin in cardioprotection against doxorubicin-induced oxidative stress, without compromising anti tumor effect of doxorubicin. Further, chronic consumption of Se-rich Spirulina phycocyanin powerfully prevents the development of atherosclerosis (Riss et al., 2007). Phycocyanin is also reported for its role as a potential therapeutic for plaque localization and its regression (Morcos et al., 1988).
Acute lung injury (ALI) is a severe complication, and characterized by damage to the epithelial and endothelial cells in lungs, thereby increasing pulmonary vascular permeability, pulmonary edema, and sequestration of polymorphonuclear neutrophils (PMNs), which finally impairs respiratory function. It is generally accepted that ALI is an excessive uncontrolled inflammatory response mediated by several pro-inflammatory mediators. Lipopolysaccharide (LPS), a cell wall component of gram-negative bacteria, is thought to play a key role in the development of ALI through stimulation of recruitment of inflammatory cells into lungs and production of several inflammatory and chemotactic cytokines. Phycocyanin exhibits an anti-inflammatory activity by inhibiting inducible nitric oxide synthase (iNOS) expression and NO production possibly by suppressing nuclear transcription factor-kB (NF-kB) activation, a key transcription factor promoting proinflammatory gene expression. Leung et al., 2013 demonstrated that posttreatment of ALI model with CPC significantly reduces the tissue permeability, and protein concentration in bronchoalveolar lavage fluid (BALF) and improves pulmonary histological alterations.
Phycocyanin has been shown to increase the expression of essential enzymes and biochemicals related to the balanced function of liver and Kidney. Phycocyanin has been documented to modulate the activities of Cyt p-450, Superoxide Dismutase, Catalases, Alanine Transaminases, Aspartate Transaminases (Ivanova et al 2010).
Human skin colour curtail from the epidermis where the pigment-producing cells melanocytes are localized to produce melanin. Its synthesis begins with catalysation of the substrates L-phenylalanine and L-tyrosine to produce L-DOPA via phenylalanine hydroxylase (PAH), tyrosinase and partly tyrosinase hydroxylase 1 (TH-1) (Gillbro JM and Olsson MJ, 2010). When ultraviolet rays penetrate the skin and damage DNA, thymidine dinucleotide fragments from damaged DNA will trigger melanogenesis and cause the melanocyte to produce melanosomes, which are then transferred by dendrite to the top layer of keratinocytes (Ellar et al., 1997). Although melanogenesis is necessary for the prevention of DNA damage and cancer caused by UV irradiation, excessive accumulation of melanin can also cause melanoma (Kawano et al., 2007).
Phycocyanin is able to serve as a potential melanogenesis inhibitor as it effectively restrained the expression of tyrosinase, the rate-limiting enzyme of melanogenesis, through the regulatory mechanisms at transcriptional (through p38 MAPK pathway on CREB activation) and post-translational (through MAPK/ERK pathway on MITF phosphorylation/degradation) levels. This phycobiliprotein exerted combinatory activities including antioxidative capacity and the regulative ability of tyrosinase expression to modulate melanogenesis (Wu et al., 2011).
Wound healing is a fundamental response to tissue injury that involves a complex set of cellular, physiological, and molecular events targeted toward the restoration of the structural and functional integrity of the damaged tissue.
Homeostasis, Inflammation, Proliferation of Keratinocytes & Fibroplast and Tissue remodeling/regeneration are the four steps involved in wound healing. Phycocyanin directly enhances wound repair by its anti-oxidant and scavenging destructive free radicals mechanism. Secondly, stimulation of keratinocyte is one mechanism by which PSE might enhance wound repair process (Gur et al., 2013).
An intake of 2 tablets of 200 mg, per day per person is widely recommended or as directed by your physician.
Blood in our body is composed of three types of cells which are suspended in liquid plasma:
Leukemia is a malignant neoplasm of the hematopoietic stem cells characterized by diffuse replacement of the bone marrow and/or peripheral blood by neoplastic cells (Prasad et al., 2013).
In leukemic condition body starts to produce more white blood cells, which are immature and do not function properly.
Symptoms of leukemia
Phycocyanin intake is accounted to be beneficial in leukemia as its administration is reported to enhance Bone Marrow Reproduction, Thymus Growth and Spleen Cell proliferation in animal models. It is also helpful in stimulation of hematopoiesis especially erythropoiesis i.e. red blood cells production (Gershwin and Belay, 2007). It is reported to exert its anticancer potential in human myeloid leukemia K-562 cells by inhibiting the proliferation of these cancer cells (Liu et al., 2000) and inducing apoptosis to them by lowering Bcl-2/Bax ratio, DNA fragmentation & PARP cleavage (Subhashini et al., 2004).
Hence Phycocyanin intake could be represented as potential candidates for leukemic patients. Nutraculture™ Phycocyanin™ comes as 200 mg tablets. An intake of 2 tablets of 200 mg each daily is widely recommended or as directed by your physician.
Liver is located at the right side of the stomach under the right rib.
Hepatocellular carcinoma or Liver cancer is one of the most common malignancies worldwide, particularly with a relatively higher incidence rate of more than 20 – 150 cases per 1, 00,000 per year in certain Asian and African areas (Shen et al., 2005). One of the major risk factor for liver cancer is alcohol. It can cause liver cirrhosis, which is the most common precursor to liver cancer. The magnitude of liver cancer is stronger if you are infected with the hepatitis B or C virus along with alcohol consumption (McKillop and Schrum, 2005). Although surgery and liver transplantation provide better outcomes, most patients are not candidates due to advanced disease, lack of donor availability, or presence of various disorders (Narvaez-Lugo et al., 2007). Further, in view of serious side effects and resistance of liver cancer towards chemotherapy there is need of shifting toward natural compounds without undesirable effects.
Phycocyanin intake is accounted to be beneficial in liver cancer as it stimulates immune system and its antioxidant profile helps in reduction of oxidative stress. Phycocyanin is also reported to inhibit Multi Drug Resistance (MDR) of human hepatocellular carcinoma Hep-G2 cell line towards doxorubicin through downregulation of reactive oxygen species and cyclooxygenase-2. It increases the accumulation of doxorubicin in doxorubicin resistant cells and decreases the PGE-2 levels (Nishanth et al., 2010). One another study reported the antiproliferation effect of c-phycocyanin aginst liver cancer Hep-G2 cell line and induction of apoptosis by interaction with membrane associated β-tubulin and glyceraldehydes-3- phosphate dehydrogenase (GAPDH), causing polymerization of microtubules and actin filaments leading to G0/G1 cycle arrest (Shanab et al., 2012).
Hence, Phycocyanin could be the new potential anticancer drug for therapy of Human Liver Cancer. Nutraculture™ Phycocyanin™ comes as 200 mg tablets. An intake of 2 tablets of 200 mg each daily is widely recommended or as directed by your physician.
Colon cancer is a neoplastic disease of the large intestine which begins at a structure called the caecum, located in the right lower quadrant of the abdomen, and continues through all portions of the abdomen to its junction with the rectum, located in the deep pelvis. It can be originated from both inherited and somatic genetic alterations that develop over the course of a lifetime (Yeatman, 2001).
Further, Cytokines and transcription factors contribute toward carcinogenesis by stimulating levels of reactive nitrogen species, activating antiapoptotic pathway and tumor progression. COX-2 over expression additionally leads to resistance of apoptosis, which enhance metastasis of colorectal cancer. PTEN is an important negative regulator of cell survival signalling and its role is the downregulation of Akt, which promotes cell survival & proliferation. Colorectal cancer cells have decreased expressions of PTEN (Saini et al., 2012).
In a recent study by Thangam et al., 2013, phycocyanin induced nuclear apoptosis accompanied by G0/G1 cell arrest and DNA fragmentation in human colon carcinoma HT-29 cells. Anticancer potential of Phycocyanin has been studied extensively by Shankar N Sanyal’s research group at the Punjab University, Chandigarh against induced tumor in animal models. Apoptosis was observed in phycocyanin treated animal through mitochondrial pathway via activation of caspases and Bcl-2 downregulation (Saini and Sanyal., 2012). Moreover, phycocyanin was evaluated individually and also adjuvant to some other anticancer molecule. It decreases expression of proinflammatory cytokines, transcriptional factors, COX-2 and thus reduced PGE-2 level demonstrated its chemopreventtive role (Saini et al., 2012). It upregulates the expression of PTEN in colonic tissues and thus inhibit PI3/Akt pathway (Saini and Sanyal., 2012). Thus, it was observed that it enhanced the anticancer effect of the molecule along with demonstrating its own anticancer potential. Result illustrated that combinatorial studies can provide significant improvement in safety and effectiveness over the monotherapy regimens. A combination of two drugs may restrain precancerous colon polyps, opening a new possible opportunity for chemoprevention of colon cancer
Hence, Phycocyanin could be the new potential anticancer drug for therapy of Human Colon Cancer. Nutraculture™ Phycocyanin™ comes as 200 mg tablets. An intake of 2 tablets of 200 mg each daily is widely recommended or as directed by your physician.
Breast cancer originates from breast cells and usually begins from the inner lining of milk ducts (ductal carcinoma) or the lobules (lobular carcinoma) that provide them milk.
Phycocyanin effect as a photosensitizer in Photodynamic therapy in case of human breast cancer cell line MCF-7 was studied (Li et al. 2010). It was observed that Phycocyanin is an ideal photosensitizer which accumulates in tumor tissue and attracts He-Ne laser to target at cancer tissues. Since phycocyanin is a natural pigment having no toxic effects, it was suggested to be a good substitute to highly toxic conventional photosensitizers or chemotherapeutic drugs. In addition to working as photosensitizer, it causes inhibition of MCF-7 cell proliferation and morphological changes like blebs formation, chromatin condensation and loss of microvilli. Phycocyanin was able to induce apoptosis by downregulation of antiapoptotic Bcl-2 proteins, NF-kB factors and activation of caspase 9.
Hence, Phycocyanin could be the new potential anticancer drug for therapy of Human Breast Cancer. Nutraculture™ Phycocyanin™ comes as 200 mg tablets. An intake of 2 tablets of 200 mg each daily is widely recommended or as directed by your physician.
Cervical cancer occurs due to uncontrolled growth of cervix cells.
Phycocyanin from S. Platensis was evaluated to study its mechanism of action against Human cervical cancer cell line HeLa (Li et al. 2006). Treatment of HeLa cells with Phycocyanin increased the percentage of hypodiploid population of cells. DNA fragmentation was also observed in treated cells. Phycocyanin was able to reduce the levels of antiapoptotic proteins like Bcl-2 and promote the expression of death receptor genes like Fas/FasL and ICAM. Caspases play a central role in virtually all known apoptotic signalling pathways and a higher level of caspase cascade was analysed in phycocyanin treated cells. There was translocation of mitochondrial cytochrome c to cytoplasm in response to apoptosis. Moreover, electron-micrographs of treated cancer cells also revealed the characteristic apoptotic features.
Hence, Phycocyanin could be the new potential anticancer drug for therapy of Human Cervical Cancer. Nutraculture™ Phycocyanin™ comes as 200 mg tablets. An intake of 2 tablets of 200 mg each daily is widely recommended or as directed by your physician.
Prostate cancer is the abnormal growth of prostate cells. There are various types of cells in prostate, but in majority of cases cancer starts in gland cells. This kind of cancer is known as adenocarcinoma. One another type of cancer is small cell carcinoma.
Antineoplastic activity of Phycocyanin was evaluated against prostate cancer cell line LnCap in combination with already known anticancer drug Topotecan (Gantar et al., 2012). It was observed that when only 10% (1µM) of the regular dose of Topotecan was combined with Phycocyanin, the cancer cells were killed at a higher rate than when Topotecan was used alone at full dose (10µM). One of the possible causes of cell death during Phycocyanin– Topotecan pooled treatment is highest levels of ROS production, leading to caspase-9 and caspase-3 activation and further DNA fragmentation. Thus, Phycocyanin working as adjuvant therapy to Topotecan increases the efficacy of drug and also reduces the amount of dose to be taken.
Hence, Phycocyanin could be the new potential anticancer drug for therapy of Human Prostate Cancer. Nutraculture™ Phycocyanin™ comes as 200 mg tablets. An intake of 2 tablets of 200 mg each daily is widely recommended or as directed by your physician.